The Cell Separation Company
Specific cell isolation within 10 min
from whole blood, buffy coat and tissue
using pluriBead
Components
Separation Consumables
Our separation system consists of three consumable parts:
- A strainer (sieve) to retain the pluriBeads and the bead bound targets
- A connector ring to detach the targets
- A funnel to support the sample loading onto the strainer
pluriStrainer
The pluriStrainer is a sterile sieving device which retains targets labeled with pluriBead. It fits into a standard 50 ml centrifuge (conical) tube that is used as a collecting container for the flow through (the detached targets, the depleted fraction or waste and unwanted targets).
pluriStrainer also enables:
- Preparation of a single cell suspension
- Preseparation for buffy coats
- Filtering of bone marrow
- Filtering of agglutinative proteins
Connector ring
Our connector ring supports the direct detachment of targets on the sieve. The sterile device is compatible with nearly all 50ml standard (conical) tubes.
Strainer, connector ring and a 50ml tube can be combined to a unit. This unit allows for a controlled flow through the sieve.
When the Luer-lock is closed, the sample remains on the sieve. An open Luer-lock starts the separation process. A pump connected to the Luer-lock supports the flow through the strainer.
The unit enables:
- detachment of the targets from the beads directly on the strainer
- lyses of the targets to extract RNA, DNA or proteins directly on the strainer
- controlled flow through the strainer with a pump device or pipette
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| Fig.: Attach the connector to a 50ml centrifuge tube. | Fig.: Attach pluriStrainer to the connector. | Fig.: If the Luer-Lock is closed, the liquid remains on the sieve. |
Funnel
The sterile funnel can be employed to support the sample loading onto the pluriStrainer. This way, larger sample volumes can be placed on the sieve and the maximum application volume of liquid increases from 4ml to 15ml.
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| Fig.: Attach the funnel to the strainer | Fig.: Funnel supported sample loading |
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From our FAQ:
8. Is it possible to simultaneously isolate more than one target from my sample?
Yes, absolutely! This is what is special about pluriSelect’s isolation technology. Currently, two different targets (cells and/or proteins) can be isolated from one sample in one single step. The target specific separation beads are mixed with 300 µl to 15 ml of sample volume in the mixing container and incubated at room temperature for 15-30 minutes. During incubation, the targets bind to their corresponding antibodies. As the beads for each target differ in their distinct size, the mixture can be separated by a sieving cascade with a bead-specific mesh size. After washing, the targets are isolated for further use. For more details please see our Technology page.