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Isolation of cells from whole blood
for RNA protein analysis
within 10 minutes
Welcome to the website of pluriSelect - a biotech company specialized on non-magnetic cell separation technology called pluriBead.
Feel free to check out our products page, or find out more about the technology.
With pluriBead® we offer a robust isolation technology for targets from different biological fluids. Throughout the development process we focused our attention on a simple but important rule: the ease of use. The design of our ready-to-use kits reflects this characteristic of our product: it is easily applicable in your daily laboratory routine.
Isolate single cells from suspensions such as Buffy Coat, cell cultures or directly from whole blood. No additional purification of the sample material is necessary. The pluriBead catcher particles are directly mixed with the sample material and bind to the target cells. Employing a sieve-separation system the bound target cells are carefully separated from unbound material. Subsequently, the target cells are detached from the catcher particles under physiological conditions within 10 minutes. The target cells are now available as a single cells suspension and can be used for further experiments such as cell culture experiments, direct stimulation, or FACS analysis. For example, isolating CD3 cells from whole blood or Buffy Coat, you obtain single cells in 30 minutes.
RNA isolation of specific cells: you can isolate cells from complex biological solutions (whole blood or cord blood) for PCR analysis in less than 10 minutes.
Activation of a cell sub-population: You can isolate your target cells and stimulate them with various concentrations of cell activators or for different activation durations. Cell stimulation can be performed direct after the cell isolation. With our pluriStrainer and connecting ring you can stimulate your cells for just a few seconds (the stimulant is simply washed off; centrifugation is not required) or serveral minutes. It is possible to control the incubation time. Because of the fast and simple cell isolation, several experiments can be performed simultaneously. As a quick rinse of cells is sufficient and centrifugation is not required at any step of the procedure even non-adherent cells can be activated in this way using pluriBead®.
In case you handle only very small or particularly precious sample material simultaneous multi-target isolation is the method of choice. Sample volumes of just 300 µl are sufficient for multiple target isolation experiments. Again, sample preparation is not necessary!