The Cell Separation Company
Specific cell isolation within 10 min
from whole blood, buffy coat and tissue
using pluriBead
Multi Target Separation
Multi Target Cell Separation - pluribead Cascade
Within the cascade mode, sieves are piled so that the top sieve holds back the bigger beads, while the lower sieve holds back the smaller beads with the respective target cells. Thus, for example, CD4 and CD8 cells can be separated from sample material simultaneously.
Three steps characterize the sorting procedure:
- Mix pluriBeads and sample material
- Pour mixture onto piled sieves, wash off unbound and unwanted cells
- Split the cascade and recover your single cell suspension
A special sample preparation is not required.
Separation scheme of a multi target cell separation
| 1. Incubation | 2. Isolation & Washing | 3. Detachment & Separation |
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| Sample and beads with different size and specificity are mixed and incubated at room temperature on a roller mixer or pluriPlix. The target cells will bind to the beads. | The target cells – now bound to the beads – are separated with pluriSelect‘s cascade technology. With target cells rosetted beads remain onto the sieve. The upper sieve holds back the big pluriBeads, while the lower sieve holds back the small pluriBeads. Washing removes all unspecific contamination. | Split the sieves. Detach cells from the beads with a detachment buffer. The cells will be released from the beads directly onto the sieve. Bead free cells will run into tube, beads remain onto the sieve. |
Cell Separation - Product Generator
For finding a match of compatible Kits use our product generator.
From our FAQ:
12. Cell stress: how much lower is the apoptosis rate and why is it so much lower compared to systems currently available
The cell stress is mainly reduced because of two inherent characteristics of the isolation kit: natural environment and minimal handling time. The target cells remain in their natural environment such as whole blood or cell culture solutions. Additionally, the number of steps or processes such as pipetting, cooling, centrifugation or changes to the cells' environment (Ficoll™ density centrifugation!) are obsolete or reduced to a minimum. This results in minimal mechanical sheer stress and the isolation duration is kept to a minimum. The isolated cells can recover in cell culture medium shortly after the isolation or can be used in other assays.