pluriBead

pluriBead® - Cell Separation


With pluriBead® we offer a robust cell isolation technology for targets from different biological fluids. Throughout the development process we focused our attention on a simple but important rule: the ease of use. The design of our ready-to-use kits reflects this characteristic of our product: it is easily applicable in your daily laboratory routine.

Find a detailed list on our products page.

pluribead - Features

No centrifugation required

No Centrifugation Required


In contrast to other cell separation methods, the pluriBead® technology does not require time-consuming centrifugation steps. This saves the highly sensitive cells from mechanical stress and keeps them vital.

Within 10-30 minutes

pluriBead® allows adapting incubation time to the user’s needs.

  • Example 1: If only 50-60% of the target cells are needed, an incubation time of 10 min. generates satisfactory isolation results.
  • Example 2: If target cells are needed for RNA/DNA/Protein isolation, they can easily be processed directly from the pluriStrainer without detaching them from the beads. Thus, incubation with detachment buffer can be omitted completely.

Non-magnetic

Non Magnetic

pluriBead® gets along without magnets. To isolate target cells from sample material, pluriBead® combines the following methods:

  • Biological: antigen - antibody - interaction to bind specific targets to catcher particles (beads)
  • Physical: filtration / sieving to isolate the bead-bound targets from the rest of the sample
  • Physiological: antigen - antibody - separation to detach targets from the catcher particles 

The catcher particles cannot be phagocytized. They can be used at room temperature and even at 37°C.

Ready 2 go

Ready To Go

pluriBead® is designed as ready-to-use kits. These contain all materials needed for your first cell separation experiment (except sample material). 

  • pluriBeads
  • Buffers (Washing buffer, Detachment buffer)
  • Consumables (pluriStrainer, Connecting Ring, Funnel)

Each of these components can be ordered separately if needed. Additionally required materials are standard laboratory equipment only (disposable globes, tubes, pipettes, roller mixer).

Up to 98% purity

Up To 98% Purity

Single cell suspensions produced with pluriBead® are devoid of any additional particles. They achieve a purity of more than 98% as the following example shall illustrate.

Fig.1 shows a FCS-SSC-Dotblot of a human buffy coat. The buffy coat was used to isolate CD3+ cells with anti-human CD3 pluriBeads. Fig.2 shows a FSC-SSC-Dotblot of enriched CD3+ cells after a positiv cell isolation via anti-human CD3 pluriBeads. The enriched cells were measured with same settings for the starting material in Fig. 1. The settings for the threshold were reduced. Fig.3 shows the enriched cells stained with CD3-FITC and CD45-PerCP. 99% auf the enriched CD3+ cells are located in the upper right quadrant.
Fig. 1: Starting Material Fig. 2: Enriched CD3 Cells Fig. 3: Enriched CD3+ Cells
99% purity of WBC-Gate