Single Target Separation (STS)

STS is an easy and fast way to isolate a specific cell type directly out of your sample material. Three steps characterize the sorting procedure:

1. Mix pluriBeads and sample material
2. Wash off unbound and unwanted cells
3. Recover your target cells

A special sample preparation is not required.

For the sorting of cell subsets which have double specificity please inform here: 
cSTS - coupled single target separation.

Separation scheme of a single target cell separation

1. Incubation	2. Isolation	3. Detachment
Sample(A) and beads(B) are mixed and incubated at room temperature on a roller mixer or pluriPlix. The target cells will bind to the beads.	The target cells – now bound to the beads – are separated with pluriSelect‘s unique sieving technology. With target cells rosetted beads remain on the strainer - washing removes all unspecific contamination.	Target cells are detached from the beads directly on the strainer with a detachment buffer. They are then washed into the tube while the depleted beads remain on the strainer.

Sample materials

• Whole Blood
• Buffy Coat
• Cell Culture & Tissue

Further applications

Replace density gradient centrifugation with pluriBead. Use specific separated cells to isolation a subpopulation for example CD4 preseparation followed by CD25 isolation.

• Positive Selection
• Depletion
• Sequential sorting

Coupled Single Target Separation

The coupled STS is a separation method to separate cells that have a double specificity. Using pluriBead in combination with any standard magnetic separation system used in your lab allows you to free your target cell population from unwanted cells and subpopulations. Use pluriBead instead of density centrifugation and indirect magnetic labeling to deplete unwanted cells. For further information, do not hesitate to contact our pluriSelect team.