Our cell separation technology

Fast target isolation using pluriBead

You need a pure cell or protein population for your experiments? You like to get your targets fast and want a fuss-free isolation system? On the following pages you will learn more about the principle of the pluriBead technology.


Here is a little introduction video how to use our technology

The isolation procedure

Our cell separation method consists of five essential steps.

Starting the separation

To start the cell isolation add pluriBead (A), with a target specific surface coating, to your sample material (B). To start the isolation mix pluriBead with your sample material.

Incubation

Sample and beads are mixed and incubated at room temperature on a roller mixer or pluriPlix. The targets will bind to the beads. Sample and beads are mixed and incubated at room temperature on a roller mixer or pluriPlix. The targets will bind to the beads.

Isolation & Washing

The targets – now bound to the beads – are separated with pluriSelect‘s unique sieving technology. With target cells rosetted beads remain onto the sieve - washing removes all unspecific contamination. The targets – now bound to the beads – are separated with pluriSelect‘s unique sieving technology. With target cells rosetted beads remain onto the sieve - washing removes all unspecific contamination.

Detachment

Detach the targets from the beads with a detachment buffer. The targets will be released from the beads directly onto the sieve. Detach the targets from the beads with a detachment buffer. The targets will be released from the beads directly onto the sieve.

Lysis (alternative to the detachment)

As an alternative to the detachment the cells can be lysed directly onto the sieve. The lysate can then be used for the isolation of RNA, DNA or proteins. As an alternative to the detachment the cells can be lysed directly onto the sieve. The lysate can then be used for the isolation of RNA, DNA or proteins.

Separate the targets from the beads

In the last step of the cell isolation cells and beads are separated. Bead free cells will run into tube, beads remain onto the sieve. Bead free cells will run into tube, beads remain onto the sieve.

Application examples for your lab

  • Single Target Cell Isolation
  • Simultaneous Isolation of several specific cell types
  • RNA Isolation
  • DNA Isolation
  • Protein Isolation
  • Cell Activation
  • Cell Depletion
  • ELISA
  • Immunoprecipitation