Lympho Spin Medium / PBMC Spin Medium

¥ 176.00
所有价格不含税,不含运费。
用于体外分离新鲜人外周血和水牛皮的PBMCs(含淋巴细胞和单核细胞的外周血单核细胞)。
SKU
60-00092
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详细内容

PBMC Spin是一种无菌的、随时可使用的密度培养基,用于从新鲜的人血或水皮中分离外周血单核细胞,产量高。用PBMC Spin培养基进行一步密度梯度离心即可富集细胞。通过与Leuko Spin Medium结合使用,在双梯度离心法中,可以在一个步骤中分离和分离粒细胞和PBMCs,具有高产率和高纯度。PBMC Spin培养基可替代Ficoll-Paque1、Pancoll2和Lymphoprep,而无需改变现有方案。富集的细胞可用于广泛的下游应用,如MACS、pluriBead或干细胞研究。

1 Ficoll-Paque是GE Healthcare/Cytiva的商标
2 Pancoll是PAN-Biotech GmbH的商标
3 Lymphoprep是Alere Technologies Inc的商标
4 MACS是Miltenyi Biotec GmbH的商标

应用

Separation scheme for PBMC enrichment with PBMC Spin Medium

At first carefully layer the sample material on top of the PBMC Spin Medium. Avoid a mixing of the two phases. Alternatively it’s possible to use pluriMate tubes. The barrier prevents the mixing of the two phases and enables to pour off the enriched cells after the first centrifugation step. After the density centrifugation aspirate the upper layer (Plasma and dilution buffer). Afterwards transfer the mononuclear cell layer to a new conical tube and wash the cells.


PBMC preparation with Lympho Spin Medium. The sample material will be overlayed on top of the density medium.

Fig. 1: Preparation of PBMC with Lympho Spin Medium. The sample material will be overlayed on top of density gradient medium.

Whole blood after density gradient centrifugation with Lympho Spin. The PBMC can be found on top of the Lympho Spin Medium.

Fig. 2.: Layers after density gradient centrifugation. The PBMC can be found on top of the Lympho Spin Medium. Erythrocytes, Granulocytes and dead cells will pass the medium and can be found at the bottom of the tube.

更多信息

更多信息
交付时间1-2
样品料Whole Blood, PBMC, Buffy Coat, Cord Blood, Bone Marrow, Primary Cell Solution
储存条件室温,避光
规范性声明仅供研究使用。不用于诊断程序。
相位范围6.8 – 7.4

数据

PBMC Spin Medium - Flow cytometry

Fig. 1: The picture of the flow cytometry analysis shows CD45+ peripheral blood mononuclear cells (PBMC) after density gradient centrifugation using PBMC Spin Medium from pluriSelect.

PBMC Spin Medium - comparison of cell sorting result with competitors

Fig.2: The composition of CD45+ PBMC after density gradient centrifugation with competitive density media using manufactor’s suggested protocol shows comparable results in fluorescence activated cell sorting analysis.

PBMC Spin Medium - yield comparison with other density gradient media

Fig. 3: The yield of PBMC using competitive density media shows no significant differences

著作