Monocytes Spin Medium

¥ 144.00
所有价格不含税,不含运费。
用于从新鲜外周血和水牛皮中体外富集单核细胞。
SKU
60-00095
Order within 34min for same day dispatch. Delivery between 2024年11月25日 and 2024年11月26日. Shipping from 德国.
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详细内容

单核细胞自旋培养基是一种即用型无菌密度培养基,用于通过单步密度离心从新鲜外周血或水样皮层中富集高产率的单核细胞。根据样品的不同,其纯度可达到60-80%。Monocytes Spin是一种温和的替代珠子介导的单核细胞富集方法,特别是对于下游应用,如细胞培养和分化。

更多信息

更多信息
交付时间1-2
样品料Whole Blood, PBMC, Buffy Coat, Cord Blood, Bone Marrow, Primary Cell Solution
储存条件室温,避光
规范性声明仅供研究使用。不用于诊断程序。
相位范围6.8 – 7.4

数据

Enrichment with Monocytes Spin Medium
Monocytes with regular PBMC medium
Enriched cells with Monocyte Spin Medium
Monocytes in CD45 population
Enriched cells with Lympho Spin Medium
Monocytes in CD45 population

Fig 1.: Enriched cells with Monocyte Spin Medium.

FSC/SSC plot of CD45+ cells after enrichment through density gradient centrifugation with Monocyte Spin Medium.

Fig 2.: Monocytes in CD45+ population.

huCD14+ plot gated on huCD45+ shows enrichment of monocytes up to 70% after density gradient centrifugation with Monocyte Spin Medium.

Fig 3.: Enriched cells with Lympho Spin Medium.

FSC/SSC Plot of CD45+ cells after enrichment through density gradient centrifugation with PBMC Spin Medium. typically PBMC consist of 70-90% lymphocytes and 10-25% monocytes. In older blood contamination with granulocytes are possible.

Fig 4.: Monocytes in CD45+ population.

huCD14+ plot gated on huCD45+ cells shows that 24,5% of the PBMC Spin enriched cells are monocytes. This proportion depends on the individual.

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