Lympho 24+ Spin Medium / PBMC 24+ Spin Medium

¥ 144.00
所有价格不含税,不含运费。
用于体外分离12小时以上的人外周血和水牛皮中的PBMC(含有淋巴细胞和单核细胞的外周血单核细胞)。
SKU
60-00093
Delivery between 2024年11月26日 and 2024年11月27日. Shipping from 德国.
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详细内容

PBMC Spin 24+ Spin是一种即开即用的无菌密度培养基,用于通过单步密度梯度离心从8-24小时的样本材料(外周血、外皮)中分离PBMC。与PBMC Spin培养基相比,PBMC24+的使用减少了在分离8小时以上的血液中发生的粒细胞和红细胞的时间依赖性污染。使用PBMC24+ Spin,即使在取样24小时后,仍可获得高纯度和高达80%的产量。富集的细胞可用于广泛的下游应用,如磁激活细胞分选(MACS)或干细胞研究。MACS是Miltenyi Biotec GmbH的商标。

应用

Separation scheme for PBMC enrichment with Lympho 24+ Spin Medium

At first carefully layer the sample material on top of the Lympho 24+ Spin Medium. Avoid a mixing of the two phases. Alternatively it’s possible to use pluriMate tubes. The barrier prevents a mixing of sample material and density solution. Furthermore the tube enables pouring off the enriched cells after the first centrifugation step. After the density centrifugation aspirate the upper layer (Plasma and dilution buffer). Afterwards transfer the mononuclear cell layer to a new conical tube and wash the cells.


PBMC preparation with Lympho 24+ Spin Medium. The sample material will be overlayed on top of the density medium.

Fig. 1: Preparation of PBMC with Lympho 24+ Spin Medium. The sample material will be overlayed on top of density gradient medium.

Whole blood after density gradient centrifugation with Lympho 24+ Spin. The PBMC can be found on top of the Lympho 24+ Spin Medium.

Fig. 2.: Layers after density gradient centrifugation. The PBMC can be found on top of the Lympho Spin Medium. Erythrocytes, Granulocytes and dead cells will pass the medium and can be found at the bottom of the tube.

更多信息

更多信息
交付时间1-2
样品料Whole Blood, PBMC, Buffy Coat, Cord Blood, Bone Marrow, Primary Cell Solution
储存条件室温,避光
规范性声明仅供研究使用。不用于诊断程序。
相位范围6.8 – 7.4