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pluriSpin® Human Monocyte Depletion is designed to isolate pure, viable and untouched monocytes-free population from whole blood, buffy coat, cord blood or similar sample material by negative isolation. The product labels monocytes, while the isolated human monocytes-free population are left in the sample. When centrifuged over a density medium the unwanted cells pellet along with the RBCs. The purified monocytes-free population are present as a highly enriched population at the interface between the plasma and the density medium.
More Information
More Information
Species
Human
Cell Types
Monocytes
Sample Material
Whole Blood, PBMC, Buffy Coat, Cord Blood, Bone Marrow, Primary Cell Solution
Purity
>90%
Storage Condition
Room Temperature
Regulatory Statement
For research use only. Not for use in diagnostic procedures,
Data
Fig 1.: CD14 in PBMC.
Fig 2.: Depleted CD14 cells using pluriSpin® Human Monocyte Depletion
Starting with fresh peripheral blood, the CD14+ cell content of depleted fraction is typically 0.2% -1%.