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TwinSpin centrifugation tubes can be filled with Density Gradient Medium (DGM) for an optimal separation of cells from whole blood and bone marrow. The TwinSpin is comprised of a standard 15 ml and an inner tube. The inner tube has an open bottom which is submersed in the DGM. Diluted anti-coagulated blood or bone marrow can simply be pipetted into the TwinSpin. The sample lays on top of the DGM inside the inner tube. During centrifugation, Leukocytes, lymphocytes and PBMCs are separated from unwanted erythrocytes and granulocytes, depending on the DGM. The result is an inter-phase enriched with target cells above the DGM.
The erythrocytes will sediment through the density gradient media out of the inner tube at the bottom of the outer tube. When separation is complete, just remove the inner tube. The elastic cap functions as valve. By pushing the cap down, the collection tube becomes a pipette. The contents can be collected drop by drop.
Bundle TwinSpin tubes with DGM of your choice.
DGM explanation
Leuko Spin - For the isolation of leukocytes from whole blood or bone marrow Lympho Spin - For the isolation of PBMC from whole blood or bone marrow Lympho Spin 24+ - For the isolation of PBMC from whole blood older than 12 hours or bone marrow PLT Spin - For the isolation of platelets from whole blood or bone marrow
Aplicación
Protocol for the use of TwinSpin® in combination with density gradient media.