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The pluriStrainer® is a sieving device (or cell strainer) for a wide range of laboratory applications where the filtration and the purification of liquids is required. Its unique design features improved ventilation to avoid clogging for a smart sample preparation. pluriStrainer® are stackable to allow direct filtration with different mesh sizes and alsocan be inverted to recover the sieved material. For large sample volumes it can be combined with a funnel. It fits all major brand 50 mL centrifuge (conical) tubes. The combination of the pluriStrainer® and the Connector Ring allow using low pressure which supports the filtration. Furthermore it is possible to block the flow through the sieve and hold back the liquid on top of the mesh. The liquid on top of the mesh can be used for sample preparation and incubation such as tissue dissociation or the treatment of cells with cell lysis reagents (e.g. Buffer RLT, TRIzol®).
Features
Filter Attach pluriStrainer® to a sterile 50 mL centrifuge tube. Then, add sample material onto the strainer and filter sample
Reverse To obtain the larger fraction, take off the pluriStrainer®, turn it upside down onto another 50 mL tube and flush back the sample from the pluriStrainer®.
Increase sample load With Funnel
You can add up to 24 mL sample material on top.
Stack Stacking of pluriStrainer® with different mesh sizes allows for straining various cell sizes at the same time.
Flow control Allows to control the rate of flow by opening or closing the Luer-Lock, e.g. for physical dissociation of primary tissue (brain, spleen etc.).
Low pressure If you add a syringe to the Connector Ring, it is possible to force low pressure to support the straining of rough sample material while pulling the piston.
Application
Obtaining a real single cell suspension after digestion of mammary tissue and organoids
Enrichment of specific cells using pluriBead technology
Preparation of a single cell suspension of blood cells from bone marrow, pancreas, thymus, lymph nodes and others
Dissociation of cells from other primary tissue
Preparation of real single cell suspension for flow cytometry(FACS™)
Faster and easier alternative to gauze filtration
Sieving of complex and viscous liquids (in combination with Connector Ring & Syringe for vacuum)
Short time incubation of cells / cytokine (in combination with pluriBead and Connector Ring)
For research use only. Not for use in diagnostic procedures,
Legal information
Buffer RLT® is a trademark of Qiagen, TRIzol® is a trademark of Molecular Research Center, Inc., FACS™ is a trademark of BD Biosciences, Corning is a registered trademark of Corning, Inc.; EASYstrainer™ is a registered trademark of Greiner Bio One International GmbH, Falcon™ Cell Strainers is a registered trademark of Corning, Inc.; MACS® SmartStrainer is a registered trademark of Miltenyi Biotec GmbH