Density Diluent Medium

Preparation of Common Densities

To create your own density solution High Density Spin Medium and Deniuent Medium has to be mixed in appropriate quantities. You can use the table below for this calculation.

Common Density (g/ml)	Volume (ml)	Cell Types	Volume of High Density Spin Medium (ml)	Volume of Density Diluent Medium(ml)
			35	65
1.069	100	Human Monocytes	15	85
1.077	100	Human PBMC	35	65
1.094	100	Human PMNC	77.5	22.5

Ensure that both solutions have the same temperature before mixing them. The High Density Spin Medium is highly viscous and needs to be pipetted very slowly. Alternativly it is possible to weigh out the releveant amounts of the media. The required mass will be calculated with the volume and the wanted density. Stir the mixture gently approximatly five minutes. Try to avoid air bubbles, otherwise you'll have to wait until the media is clear again before starting your experiment.