pluriSpin® Human PLT Depletion (2 ml)

pluriSpin® Human PLT Depletion is designed to isolate pure, viable and untouched platelets-low population from whole blood, buffy coat, cord blood or similar sample material by negative isolation. The product labels Platelets, while the isolated human platelets-low population are left in the sample. When centrifuged over a density medium. The unwanted cells pellet along with the RBCs. The purified platelets-free population are present as a highly enriched population at the interface between the plasma and the density medium.

Problems with platelet contamination

• Reduce purity of enriched cells in fresh blood and even more if the blood sample is older than 8 hours.
• To prevent platelet activation samples containing platelets should be handled gently
• Platelets disturb your cell culture by taking space and setting free growth factors and other mediators
• Platelets can create cell clumps
• Platelets are sticky and can easily bind to other cells (i.e. monocytes, eosinophils)
• Platlets can affect FACS analysis and cause large variation of DNA content

Solution and advantages with pluriSpin® Human PLT Depletion

• Depletion without the use of beads
• Increases purity of enriched cells through a density gradient centrfugation in one step
• Can be combined with negative separation technniques such as pluriSpin®
• Rare cell enrichment will be improved (e.g. in use with pluriSpin® CD45 Depletion)
• Minimum manipulation of blood sample due to shorter centrifugation step (compared to standard protocol with platelet density gradient)
• Cell enrichment from blood samples older than 8 hours will be improved