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当社は、お客様に最適な体験を提供するためにクッキーを使用しています。 技術的に必要なクッキーはショッピングを可能にするために使用され、統計は匿名化されたGoogleアナリティクスに使用されます。 すべては、更新された プライバシーポリシー.
Leukocytes belong to the immune system and protect the body against infections and foreign substances. Leukocyte concentrates are available directly from the blood donation. For smaller volumes, it is possible to separate leukocytes or PBMC (peripheral blood mononuclear cells) via density centrifugation. Buffy Coats from a blood donation are interesting for direct cell isolation with pluriBead.
A "Buffy Coat", also known as leukocyte concentrate, is a byproduct in the manufacturing of red blood cell and platelet concentrate from a whole blood donation. The compatibility of the donated blood samples are increased due to the removal of leukocytes that are known to belong to the human immune system, increases the compatibility and the shelf life of blood products. Risks and side effects, such as fever or formation of antibodies that arise during the administration of transfusions, can be minimized by the elimination of the white blood cells. A blood bag, which is intended for medical use must not contain more than one million leukocytes. Buffy Coat is the resulting waste-product, which contains 10-20 times concentrated leukocytes.
Two ways are suitable for the preparation of buffy coat. On the one hand the whole blood donation is centrifuged to separate the whole blood in red cells, plasma and buffy coat. The second possibility is to filter the blood and hold back the leukocytes.
An additional possibility is to produce the buffy coat by yourself. Therefore just follow the short protocol: