The store will not work correctly in the case when cookies are disabled.
Cookie Settings
We use cookies to provide an optimal experience for you.Technically required cookies are used for making shopping possible, statistics are used for anonymized Google Analytics. You can read everything in our updated privacy policy.
Red Blood Cell (RBC) Lysis Buffer has been designed, formulated, and tested to ensure optimal lysis of RBCs in single cell suspensions with minimal effects on leukocytes. Nucleated RBCs are not effectively lysed with ammonium chloride. This 1x RBC Lysis Buffer is ready to use.
Components
NH 4 Cl (155 mMol/L)
KHCO 3 (10 mMol/L)
EDTA (0,1 mMol/L)
Protocol
Ensure that the RBC Lysis Buffer is cold (4-8°C) .
Dilute one volume of cell suspension with 10 volumes of 1× Red Blood Cell Lysis Solution.
When working wih blood samples: Vortex for 5 seconds and incubate for 10-15 minutes (no more than 15 minutes) at 4-8°C.
When working with tissue samples: Vortex for 5 seconds and incubate for 2 minutes at 4-8°C.
Note: Observe turbidity to evaluate red blood cell lysis. Once the sample becomes clear red, lysis is complete.
Vortex for 5 seconds and centrifuge at 300×g for 10 minutes at room temperature.
Aspirate supernatant completely.
Resuspend the cell pellet in an appropriate buffer and proceed to further applications.
추가 정보
추가 정보
Size
100 ml
Delivery Time (days)
1-2
Area of interest
Cell Biology
Sterility
Sterile
pH Range
pH 7.4
Shipping Condition
Room Temperature
Storage Condition
4-8 °C
Regulatory Statement
For research use only. Not for use in diagnostic procedures.,