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pluriSpin® Human CD4⁺ T Cell Enrichment is designed to isolate pure, viable and untouched CD4⁺ T cells from whole blood, buffy coat, cord blood or similar sample material by negative isolation. The product labels Monocytes, cytotoxic T cells, B cells, NK cells and granulocytes, while the negatively isolated human CD4⁺ T cells are left in the sample. When centrifuged over a density medium the unwanted cells pellet along with the RBCs. The purified CD4⁺ T cells are present as a highly enriched population at the interface between the plasma and the density medium.
More Information
More Information
Species
Human
Cell Types
Helper T cells
Sample Material
Whole Blood, PBMC, Buffy Coat, Cord Blood, Bone Marrow, Primary Cell Solution
Purity
>90%
Storage Condition
Room Temperature
Regulatory Statement
For research use only. Not for use in diagnostic procedures,
Data
Fig 1.: CD4 in PBMC.
Fig 2.: Enriched and untouched CD4 cells using pluriSpin® CD4+ T Cell Enrichment
Starting with fresh peripheral blood, the CD4+ cell content of the enriched fraction is typically 88% - 94%.