Leukocytes, the white blood cells,

Leukocytes, the white blood cells, are a central component of the immune system and play an essential role in the defense against pathogens and in immune regulation. They develop from haematopoietic stem cells in the bone marrow and circulate in the blood and in various tissues, where they recognize and eliminate pathogenic microorganisms, foreign substances and abnormal cells. Through complex signaling mechanisms, leukocytes can specifically migrate into infected or inflamed tissue, trigger immune reactions and contribute to tissue regeneration. Their function and activity are regulated by a finely tuned network of cytokines and other immune modulators. Dysfunctions or quantitative deviations in the leukocyte count can be associated with immunological disorders, inflammatory processes or haematological diseases.

Leukocytes can be divided into three main groups:

- Granulocytes

- Lymphocytes (T-cells, B-cells, NK-cells) and 

- Monoczytes, 

which take on specific functions in the immune response. 

The subpopulations of the individual leukocyte cell groups are extremely diverse and are subject to continuous change, as new protein structures, functions and cell interactions are constantly being discovered. In addition to histological classification, the cluster-of-differentiation (CD) nomenclature has long been used to characterize immune cells. Both the presence and absence of certain CD markers can be decisive for the differentiation of individual cell populations.

A common feature of all leukocytes is the expression of CD45 (CD45⁺). This surface protein, also known as receptor tyrosine protein phosphatase C, is exclusively expressed by all nucleated cells of hematopoiesis and is highly conserved.

Leukocytes or specific subgroups can be isolated and enriched using various methods.

pluriBead, products for positive CD45 cell separation

pluriBead Human CD45 cell separation

are suitable for rapid, positive cell separation of CD45+ cells from human sample material. 

to the  product >

pluriBead Murin CD45 cell separation

are suitable for rapid, positive cell separation of CD45+ cells from murine sample material. 

to the  product >

pluriSpin, products for negative CD45 cell separation

pluriSpin Human CD45 cell separation

are suitable for rapid, negative cell separation of CD45+ cells from human sample material. 

to the  product >

Leuko Spin Media, products for the enrichment of leukocytes through density gradient cell separation

Leuko Spin Medium

for the enrichment of leukocytes using density gradient centrifugation from fresh sample material.

to the  product >

Leuko 24+ Spin Medium

 for the enrichment of leukocytes by density gradient centrifugation from sample material more than 12 hours old.

to the  product >