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pluriSpin Human CD8⁺ T-cell Depletion

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Depletion of human CD8+ T-cells from whole blood, bone marrow, umbilical cord blood and buffy coat using negative selection. 1 vial, 2 ml

156.00 € 156.0 EUR 156.00 €

105.00 €

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Catalog No. 19-04002-31

Terms & Conditions​
All ordering information can be found here. 

HS Code: 38229000

Details pluriSpin human CD8⁺ T-cell depletion, untouched cell separation

pluriSpin human CD8+ T-cell depletion, for negative selection of cells, has been developed to isolate a clean, vital and untouched CD8+ T-cell free cell population from whole blood, buffy coat, cord blood, bone marrow or similar sample materials. The product labels all CD8+ T cells and leaves the CD8+ T cell free fraction untouched.

Subsequent PBMC spin density gradient centrifugation is used to remove all labeled cells from the sample together with the red blood cells (RBCs) and granulocytes. The untouched and unlabeled CD8+ T cell free fraction remains enriched in the interphase between the plasma and the density medium. We recommend PBMC Spin Medium as the density medium for DGM centrifugation. Alternatively, Ficoll, Lymphoprep or other comparable density media can be used without changing the protocol.

Specification​FeaturesAccesories​Support​

Specification

Target cells

Cytotoxic T cells

Cell separation method

negative

Cell depletion

Performed with PBMC Spin density gradient medium.

Compatible with

PBMC 24+ Spin Medium, Lymphoprep, Ficoll, Pancoll​


Species

Human

Sample Material

Whole blood, PBMC, PMNC, bone marrow, cell cultures

Cell separation platform

non-magnetic

Can be combined with

pluriMate or SepMate tubes

Features

Negative selection

Unwanted cells are labeled with specific antibodies and then removed, while the desired cells remain unlabeled in the suspension and retain their natural properties.

High purity and cell viability

The isolated cells remain functional and can be used directly for subsequent experiments such as flow cytometry (FACS), cell culture or molecular biological analyses.

No magnetic beads or columnsrequired

Unlike many other cell separation methods, pluriSpin does not require magnetic beads or columns, which simplifies the process and preserves cell integrity.

Compatible

Purification by density centrifugation can be carried out using PBMC Spin Medium or comparable density media from other manufacturers, such as Lymphopreo, Ficoll or Pancoll.

pluriSpin, non-magnetic negative cell separation.

Isolate. Centrifuge. Analyze.

Discover all advantages > 

Accesories

Buffers and consumables for rapid cell enrichment with pluriSpin can be found here:

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​Support

Product specification

Information
Variant 1 VIAL, 2 ml
Species Human
Cell type Cytotoxic T cells
Antigen CD8
Purity > 90%
Cell separation platform non-magnetic
Cell separation method negative, depletion
Sample material whole blood, PBMC, Buffy Coat, cord blood, bone marrow, primary cell suspension
Can be combined with PBMC Spin Medium, pluriMate separation tubes, Lymphoprep™, Ficoll™, Pancoll, SepMate™-15 ml tubes, SepMate™-50 ml tubes, Leucosep 12 ml tubes, Leucosep 50 ml tubes
General information
Item packing 1 VIAL, 2 ml / VIAL
Manufacturer pluriSelect
Terms of delivery ambient temperature
Storage condition 4 - 8 °C
Regulatory statement For research use only. Not for use in diagnostic procedures.