Specification
Target cells
platelets
Separation soul close
1,060 g/ml
Cell separation method
negative
Sample material
whole blood, Buffy Coat
Compatibility with
pluriMate, SepMate, LeucoSep
Can be used with
pluriSpin, MACS, EasySep, RosetteSep, MojoSort
Features
Enrich
PLT Spin can be used to obtain platelet concentrates from small sample volumes.
Ready to use
pluriSpin separation media can be used directly for cell separation.
Deplete
PLT Spin can be used to remove unwanted platelets from samples, such as enriched PBMC or leukocytes.
Combinable
pluriSpin separation media can be combined with each other and can be used, for example, as a double gradient for the simultaneous enrichment of two cell populations.
Separation scheme
Distribution of blood cell populations as a function of density
Blood consists of the following cell populations: thrombocytes (1), monocytes (2), lymphocytes (3), basophilic granulocytes (4), neutrophilic granulocytes (5), eosinophilic granulocytes (6) and erythrocytes (7). The cell populations differ in density and cell number. The density of the cell populations can be used for enrichment with the support of density media.
Target cell population with PLT Spin density centrifugation
The density of the separation media defines the cut-off for the cells. PLT Spin Medium has a cut-off of 1.060 g/ml. All cells with a lower density, such as thrombocytes (1), are enriched with PLT Spin Medium. All cells with a higher density, such as monocytes (2), lymphocytes (3), basophil granulocytes (4), neutrophil granulocytes (5), eosinophil granulocytes (6) and erythrocytes (7) pass through the PLT Spin Medium and are depleted.
At first, the sample material is carefully layered onto the PLT Spin medium. Mixing of the two phases must be avoided. After density gradient centrifugation, the upper layer (plasma and dilution buffer) is aspirated. The layer of platelets is then transferred to a new tube and the cells are washed.
Tip! Use pluriMate tubes.
For overlaying the density gradient medium, we recommend our pluriMate tubes. The integrated barrier of the pluriMate tubes prevents mixing of the two phases (sample material and separation medium) and allows the enriched cells to be poured off after the first centrifugation step. The tubes save time and increase the reproducibility of the results.
Before centrifugation
Preparation of leukocytes with Leuko Spin Medium. The sample material (1) is carefully placed on the Leuko Spin Medium (2).
After centrifugation
Layers after centrifugation. After centrifugation, the platelets form a white layer (2) on the PLT Spin Medium (3). Cells with a higher density, such as erythrocytes, granulocytes, lymphocytes and dead cells, pass through the medium and are located at the bottom of the tube (4).
pluriSelect, density gradient separation media.
Cell Separation. Simple. Fast. Easy.