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pluriBead Murin CD45 cell separation

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CD45 pluriBeads are suitable for rapid, positive cell separation of CD45+ cells from murine sample material. The microbeads can be used without sample preparation, for cell isolation, directly in blood (whole blood), buffy coat or cell cultures. pluriBeads are available as S- and M-pluriBeads. S and M define the size of the microbeads.

108.00 € 108.0 EUR 108.00 €

146.00 €

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  • Variant
  • Species
  • Antigen
  • Cell type
  • Sample material
  • Cell separation method
  • Cell separation platform
  • Item packing
  • Manufacturer
  • Terms of delivery
  • Storage condition
  • Regulatory statement
  • Sample material
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Catalog No. 29-04500-10

Terms & Conditions​
All ordering information can be found here. 

HS Code: 38229000

Details, pluriBead Murin CD45 cell separation

CD45 pluriBeads are suitable for rapid, positive cell separation of CD45+ cells from murine sample material. The target-specific non-magnetic pluriBeads can be used without sample preparation for cell isolation, directly in blood (whole blood), buffy coat or cell cultures. For mixing the CD45-specific pluriBeads with the sample material, we recommend our universal mixing device the pluriPlix or a horizontal mixer.

During the incubation period, the CD45+ cells from the sample bind to the specific surface of the pluriBeads. The pluriBeads, with the bound cells, are then separated from all unbound cells using a pluriStrainer. In the final step, the target cells are gently detached from the pluriBeads using our detachment buffer.

The enriched, particle-free CD45+ cells are immediately available for further downstream applications such as cell culture experiments or FACS analyses.
For the isolation of cell-specific RNA, DNA or proteins, the bound cells can be lysed directly on the pluriStrainer without the detachment step.

Specification​pluriBead TypeFeaturesAccessories​Support​

Specification

Target Cells

CD45+ cells

Cell Types

Leukocytes​

Cell Separation Method

positive

Cell Enrichment

with 30 or 60 µm pluriStrainer


Species

Mouse

Sample Material

Whole blood, PBMC, tissue, bone marrow, cell cultures

Cell Separation Platform

non-magnetic

Detachability

Yes, the CD45+ cells can be detached from the pluriBead.

pluriBead Type

pluriBead is available as S-pluriBead and M-pluriBead.
S and M define the size of the microbeads.

S-pluriBead​

are 32 µm in diameter

For a small number of target cells < 1x 10^6 cells / ml sample volume.

Further informations >
M-pluriBead​

are 65 µm in diameter

For a high number of target cells > 1x 10^6 cells / ml sample volume.

Further informations >

Features

No sample preparation

Use 200 µl - 45 ml whole blood sample material without prior density gradient centrifugation or red blood cell lysis.

Fast, gentle cell isolation

Isolate cells in just 5 minutes (depending on the cell type and the amount of target cells available).

Simultaneous cell isolation​

Combine S- and M-pluriBeads and isolate two different cell types from one sample at the same time. 

Sequential cell isolation

Unbound cells in a sample are not lost, but can be concentrated and reused for isolation.

pluriBead, non-magnetic cell separation.

Isolate. Sieve. Detach. Analyze.

All advantages > 

Accessories

Buffers and consumables for rapid cell enrichment with pluriBead® can be found here:

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​Support

Product specification

Information
pluriBead Type S-pluriBead or M-pluriBead
Variant 1 VIAL, 2 ml
Species Murin
Antigen CD45
Cell type Leukocytes
Sample material primary cell suspension, whole blood, PBMC, Buffy Coat, cord blood, bone marrow
Cell separation method positive
Cell separation platform non-magnetic
General information
Item packing 1 VIAL, 2 ml / VIAL
Manufacturer pluriSelect
Terms of delivery ambient temperature
Storage condition 4 - 8 °C
Regulatory statement For research use only. Not for use in diagnostic procedures.